Transcriptomic, histological and biochemical analyses of Macrobrachium nipponense response to acute heat stress

Temperature is an essential environmental factor affecting the viability of organisms. changes could cause damages or even death. The oriental river prawn, Macrobrachium nipponense, widely distributed in East Asian countries, and has been important economical aquaculture species China since 1990s. This prawn belongs to crustaceans, which a kind ectotherms very sensitive temperature due their relatively low internal metabolic heat production high thermal conductivity. In order reveal adaptation mechanisms, M. nipponense were acclimated at 25 °C for 24 h, successively challenged 30 35 h same tank, respectively. And histological, biochemical transcriptomic analyses carried out on gills hepatopancreas under different temperatures. results showed that degrees histological discovered both °C. digestive, metabolic, antioxidative capacity enhanced A total 2293 3304 differentially expressed genes (DEGs) identified from hepatopancreas, These DEGs significantly involved regulation transcription, proteolysis, nucleus, cytoplasm, metal ion binding, ATP binding by Gene ontology (GO) enrichment analysis. Furthermore, enriched KEGG pathways including neuroactive ligand-receptor interaction, thyroid hormone synthesis, ECM-receptor interaction. cGMP-PKG signaling pathway, ribosome, calcium pathway. be helpful further understanding molecular mechanisms underlying response increasing knowledge freshwater crustaceans context global warming.